Basic Investigations
STUDY ON ANTITUMOR DRUG-INDUCED APOPTOSIS IN HUMAN CANCER CELLS BY TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE ASSAY
Abstract
Objective: Considerable evidence has showed that apoptosis is involved in both cancer development and inhibition. A new assay (terminal deoxynucleotidyl tansferase, TdT) was recently reported to have advantages in the detection of apoptosls. In this study, this assay was used to investigate antitumor drug-induced apoptosls in bumaq cancer cells.
Methods: TdT assay, DNA gel electrophoresls, electron and light microscopy were used to observe apoptosls.
Results: Our results showed that clsplatin-induced apoptosis in both HL-60 and SV40T-transformed human bronchial epithelial cells was detected with a good dosage and time response. The occurrence of the apoptosls was preceded by the decrease of bel-2 mRNA expression. With the TdT assay, apoptotic cells were observed in ovarian tumor of patients treated with carboplatin.
Conclusion: TdT assay may be applicable to monitor apoptosis in human cancers induced by chemotherapy, and to evaluate tumor cell response during treatment.
Methods: TdT assay, DNA gel electrophoresls, electron and light microscopy were used to observe apoptosls.
Results: Our results showed that clsplatin-induced apoptosis in both HL-60 and SV40T-transformed human bronchial epithelial cells was detected with a good dosage and time response. The occurrence of the apoptosls was preceded by the decrease of bel-2 mRNA expression. With the TdT assay, apoptotic cells were observed in ovarian tumor of patients treated with carboplatin.
Conclusion: TdT assay may be applicable to monitor apoptosis in human cancers induced by chemotherapy, and to evaluate tumor cell response during treatment.
