@article{CJCR2033,
author = {Tong Tong and Hanxiao Sun and Liying Liu and Jinfeng Liang and Suping Guo and Naijun Han and Shujun Cheng},
title = {STUDY ON ANTITUMOR DRUG-INDUCED APOPTOSIS IN HUMAN CANCER CELLS BY TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE ASSAY},
journal = {Chinese Journal of Cancer Research},
volume = {9},
number = {3},
year = {2013},
keywords = {},
abstract = {Objective: Considerable evidence has showed that apoptosis is involved in both cancer development and inhibition. A new assay (terminal deoxynucleotidyl tansferase, TdT) was recently reported to have advantages in the detection of apoptosls. In this study, this assay was used to investigate antitumor drug-induced apoptosls in bumaq cancer cells.
Methods: TdT assay, DNA gel electrophoresls, electron and light microscopy were used to observe apoptosls.
Results: Our results showed that clsplatin-induced apoptosis in both HL-60 and SV40T-transformed human bronchial epithelial cells was detected with a good dosage and time response. The occurrence of the apoptosls was preceded by the decrease of bel-2 mRNA expression. With the TdT assay, apoptotic cells were observed in ovarian tumor of patients treated with carboplatin.
Conclusion: TdT assay may be applicable to monitor apoptosis in human cancers induced by chemotherapy, and to evaluate tumor cell response during treatment.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/2033}
}