Original Article
THE EXPRESSION OF CDK4 GENE IN HUMAN BREAST CANCER
Abstract
Purpose: To analyze the genetic changes of CDK4 gene and expression of CDK4 mRNA in the breast ganger.
Materials and methods: We obtained a fragment of CDK4 gene, from many kinds of human cancer or normal tissues by PCR .Taking it as a probe, using the methods of Southern and Dot blotting, we analyzed the role of CDK4 gene in the occurrence and development of human breast cancer.
Results: CDK4 gene appeared amplification and its mRNA overexpressed clearly in the breast cell line(MDA231). In human breast cancer tissues, CDK4 mRNA expressed much more than normal breast tissues(p<0.05).
Conclusion: in the tumorigenesis process, the regulative role of CDK4 is important, its abnormal amplification or expression will cause cell division cycle into a disorder one, then the cell may enter an unlimited proliferation cycle.
Materials and methods: We obtained a fragment of CDK4 gene, from many kinds of human cancer or normal tissues by PCR .Taking it as a probe, using the methods of Southern and Dot blotting, we analyzed the role of CDK4 gene in the occurrence and development of human breast cancer.
Results: CDK4 gene appeared amplification and its mRNA overexpressed clearly in the breast cell line(MDA231). In human breast cancer tissues, CDK4 mRNA expressed much more than normal breast tissues(p<0.05).
Conclusion: in the tumorigenesis process, the regulative role of CDK4 is important, its abnormal amplification or expression will cause cell division cycle into a disorder one, then the cell may enter an unlimited proliferation cycle.
