THE ANTITUMOR ACTIVITY OF ELEMENE IS ASSOCIATED WITH APOPTOSIS

Elemene, isolated from the Chinese medicinal herb Rhizoma Zedoariae was shown to exhibit antitumor activity in vitro and in vivo to human and murine tumor cells. This novel antineoplastic agent has been demonstrated to have substantial clinical activity against various tumors. In this paper, the mechanisms of antitumor activity of elemene are reported. The in vitro effect of elemene on the growth of leukemia cells was evaluated by MTT assay. The ICss values of elemenc for promyelocytic leukemia HL-60 cells and erythroleukemia K562 cells were found to be 27.5 μg/ml and 81μg/ml, respectively, while ICso for peripheral blood leukocytes (PBL) was 254.3 μg/ml. The inhibitory effect of elemene on proliferation of HL-60 cells was associated with cell cycle arrest from S to G2M phase transition and with induction of apoptosis. The apoptosis of tumor cells was confirmed by DNA ladder formation on gel electrophoresis and characteristic ultrastrnctural alternations. These results indicate that induction of apoptosls contributes to the mechanisms of antitumor activity of elemene.