Original Article
INDUCTION OF APOPTOSIS IN HUMAN GASTRIC CARCINOMA CELL LINE MGC-803 BY MONOCLONAL ANTIBODY PD4
Abstract
Objective: To study the effect of McAb PD4 on the cell cycle and on cell injury in the gastric carcinoma cell line, MGC-803.
Methods: The effects of McAb PD4 on cell proliferation cycle and cell injury of MGC-803 cells Were examined by flow cytometry analysis, DNA electrophoresis and terminal deoxynucle otidyl transferase assay. Fas antigen was investigated by ELISA.
Results: McAb PD4 inhibited tumor-growth of MGC-803 cells in nude mice by inducing apoptosis.
Conclusion: P40 is a tumor-associated antigen distinct from the Fas antigen. Molecular cloning of P40 will define the pathway and mechanism of apoptosis induced by McAb PD4. Induction of apoptosis by McAb PD4 may be a useful therapeutic approach in treating cancer.
Methods: The effects of McAb PD4 on cell proliferation cycle and cell injury of MGC-803 cells Were examined by flow cytometry analysis, DNA electrophoresis and terminal deoxynucle otidyl transferase assay. Fas antigen was investigated by ELISA.
Results: McAb PD4 inhibited tumor-growth of MGC-803 cells in nude mice by inducing apoptosis.
Conclusion: P40 is a tumor-associated antigen distinct from the Fas antigen. Molecular cloning of P40 will define the pathway and mechanism of apoptosis induced by McAb PD4. Induction of apoptosis by McAb PD4 may be a useful therapeutic approach in treating cancer.
