Basic Investigations
ANTIGEN ASSOCIATION OF J6-1 CE~LL MEMBRANE ASSOCIATED FACTOR. RECEPTOR WITH MACROPHAGE COLONY STIMULATING FACTOR RECEPTOR
Abstract
Objective: To verify the antigen association of MAF-J6-1 receptor with M-CSFR and to further study the role of M-CSF and its receptor mediated juxtaerine in promoting leukemic cell proliferation.
Methods: Monoclonal antibody (McAb) of MAF-J6-1R RE2 and polyclonal antibody (PolyAb) of rhM-CSFR were prepared. The specificity of MeAh RE2 to M-CSFR was confirmed by indirect ELISA, cross-neutralizing assay with J6-1 cell colony formation and neutralization test by ELISA.
Results: The reactive activity of purified RE2 to M-CSFR was over 1: 16000. The inhibitory activity of M-CSFR and MAF-J6-1R could be blocked by RE2 and antI-M-CSFR antibody. The reactivity of RE2 to M-CSFR could be reduced by M-CSFR.
Conclusion: The specificity of RE2 to M-CSFR was confirmed and the antigen association of MAF-J6-1R with M-CSFR was proved, It suggests that M-CSF and its receptor mediated auto-juxtaerine stimulation could be an operative mechanism in either leukemia or nonhematological malignancies.
Methods: Monoclonal antibody (McAb) of MAF-J6-1R RE2 and polyclonal antibody (PolyAb) of rhM-CSFR were prepared. The specificity of MeAh RE2 to M-CSFR was confirmed by indirect ELISA, cross-neutralizing assay with J6-1 cell colony formation and neutralization test by ELISA.
Results: The reactive activity of purified RE2 to M-CSFR was over 1: 16000. The inhibitory activity of M-CSFR and MAF-J6-1R could be blocked by RE2 and antI-M-CSFR antibody. The reactivity of RE2 to M-CSFR could be reduced by M-CSFR.
Conclusion: The specificity of RE2 to M-CSFR was confirmed and the antigen association of MAF-J6-1R with M-CSFR was proved, It suggests that M-CSF and its receptor mediated auto-juxtaerine stimulation could be an operative mechanism in either leukemia or nonhematological malignancies.
