Original Article
CLONING AND EXPRESSION OF A GENE ASSOCIATED WITH HL60 CELL APOPTOSIS INDUCED BY INHIBITION OF POLYAMINE BIOSYNTHESIS
Abstract
Objective: To done the gene assodated with apoptoas induced by an inlu%itor of polyamine biosynthesis, ¢zdifluommethylornithine (DFMO).
Methods: The differenlial subtraction screening was used for gene doning from cDNA h%rary of HL60 cells treated by DFMO. Northern blot, morphological observation, FCM assays and ladder map of DNA eleclrophoresis were performed.
Results: The transfecfing gene expression and activity of inducing apoptosis in the cells transfected from recombinant plasmid containing the cloned fragment df4 was proved.
Conclusion: It is suggest that df4 gene cloned ha the study could be a gene regulating apoptosis of HL60 cells.
Methods: The differenlial subtraction screening was used for gene doning from cDNA h%rary of HL60 cells treated by DFMO. Northern blot, morphological observation, FCM assays and ladder map of DNA eleclrophoresis were performed.
Results: The transfecfing gene expression and activity of inducing apoptosis in the cells transfected from recombinant plasmid containing the cloned fragment df4 was proved.
Conclusion: It is suggest that df4 gene cloned ha the study could be a gene regulating apoptosis of HL60 cells.
