@article{CJCR1631,
author = {Lixin Feng and Muzhen Fan},
title = {CLONING AND EXPRESSION OF A GENE ASSOCIATED WITH HL60 CELL APOPTOSIS INDUCED BY INHIBITION OF POLYAMINE BIOSYNTHESIS},
journal = {Chinese Journal of Cancer Research},
volume = {11},
number = {2},
year = {2013},
keywords = {},
abstract = {Objective: To done the gene assodated with apoptoas induced by an inlu%itor of polyamine biosynthesis, ¢zdifluommethylornithine (DFMO).
Methods: The differenlial subtraction screening was used for gene doning from cDNA h%rary of HL60 cells treated by DFMO. Northern blot, morphological observation, FCM assays and ladder map of DNA eleclrophoresis were performed.
Results: The transfecfing gene expression and activity of inducing apoptosis in the cells transfected from recombinant plasmid containing the cloned fragment df4 was proved.
Conclusion: It is suggest that df4 gene cloned ha the study could be a gene regulating apoptosis of HL60 cells.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/1631}
}