IN VITRO AND IN VIVO CHEMOTACTIC EFFECT OF MIP-lα GENE TRANSFECTED TUMOR VACCINE ON IMMUNE EFFECTOR CELLS 1

Vaccination with chemokine gene-transfected tumor cells may be a new apporach to cancer treatment. Macrohage inflammatory protein-la (MIP-lα) is a new type of chemokines which has chemotactic activity on effector cells. In the present study, the B16 melanoma cells were transfected with recombinant adenovirus harboring murine MIP-Iα gene. The biological characteristics of the MIP-1α gene transfected B16 melanoma cells were investigated. The level of MIP-lαin the supernatant of gene-transfected melanoma cells was 368 ± 24 ng/ml/10⁶/24hr. This supernatant showed strong chematactic activity for NK cells, CD4⁺ T cells, CD8⁺ T cells or the freshly isolated peritoneal macrophages. Though the in vitro growth were not altered, the tumorigenicity of the gene-transfected B16 melanoma cells decreased signicantly. The infiltration of inflammatory cells into the tumor mass formed by MIPla gene-transfected B16 cells were much more obvious than that by wild-type B16 cells or B16 cells transfected with the control gene. However, the survival time of the mice bearing BI6 melanoma cells transfected with MIP-1 α gene was not prolonged and the NK, CTL activity remianed unchanged. We suggested that the in vivo phenomenon may be related to the high toxicity of the MIP-1 α as a strong non-specific inflam-matory mediator.