Original Articles
CONGENITAL EXPRESSION OF mdr-1 GENE IN FRESH CANCER TISSUES FROM SEVERAL HIGH-INCIDENCE NEOPLASMS WITHOUT PREOPERATIVE CHEMOTHERAPY
Abstract
Objective: The purpose of the present study is to detect characteristics of primary expression of mdr-1 gene in several neoplasms which .has high morbidity in clinic.
Methods: 151 resected samples, which are pathologically malignant and clinically untreated before operation, were obtained from Anyang Cancer Hospital. All of them were investigated with RT-PCR for the expression of mdr-I gene and correlated each other. Besides, we evaluated the advantages of RT-PCR in this study.
Results: The mdr-1 gene expression rate of these 151 samples, including cancers of stomach and gastric cardia (n=51), esophagus (u--46), colorectum (n=16), breast (n=15), thyroid (n=10), lung (n=9), uterine cervix (n=4), was 33.3%, 37%, 31.3%, 13.2%, 40%, 55%, 0%, respectively.
Conclusion: Compared with other methods, RT-PCR for studying mdr-1 gene expression had certain advantages in simplicity, reliability, and accuracy. Overexpression of mdr-I gene in these neoplasms suggested that cases should be distinguished before treatment according to MDR of tumor and to choose effective drugs for individual cancer patient.
Methods: 151 resected samples, which are pathologically malignant and clinically untreated before operation, were obtained from Anyang Cancer Hospital. All of them were investigated with RT-PCR for the expression of mdr-I gene and correlated each other. Besides, we evaluated the advantages of RT-PCR in this study.
Results: The mdr-1 gene expression rate of these 151 samples, including cancers of stomach and gastric cardia (n=51), esophagus (u--46), colorectum (n=16), breast (n=15), thyroid (n=10), lung (n=9), uterine cervix (n=4), was 33.3%, 37%, 31.3%, 13.2%, 40%, 55%, 0%, respectively.
Conclusion: Compared with other methods, RT-PCR for studying mdr-1 gene expression had certain advantages in simplicity, reliability, and accuracy. Overexpression of mdr-I gene in these neoplasms suggested that cases should be distinguished before treatment according to MDR of tumor and to choose effective drugs for individual cancer patient.
