Original Article
STUDY ON THE MECHANISM OF ESCAPING IMMUNE SURVEILLANCE IN HUMAN GLIOMAS
Abstract
Objective: To study mechanisms by which human gliomas may escape immune surveillance.
Methods: The effect of supernatant (SN) obtained from cultured media of malignant glioma cell lines on the proliferation of phytohemagglutinin-p stimulated peripheral blood lymphocytes (PBLs) from healthy subjects and patients with gliomas was examined by MTT assay. The immunosuppressive factor which might be existed in the SN was identified by neutralization method with specific antibodies and Northern blot hybridization of glioma cells. In addition, the cellular immunity of patients with gliomas and relevant hormone and catecholamine were determined.
Results: It was found that the malignant glioma cells could release an immunosuppressive factor in an autocrine fashion which was further identified as the transforming growth factor β,sub>2
Methods: The effect of supernatant (SN) obtained from cultured media of malignant glioma cell lines on the proliferation of phytohemagglutinin-p stimulated peripheral blood lymphocytes (PBLs) from healthy subjects and patients with gliomas was examined by MTT assay. The immunosuppressive factor which might be existed in the SN was identified by neutralization method with specific antibodies and Northern blot hybridization of glioma cells. In addition, the cellular immunity of patients with gliomas and relevant hormone and catecholamine were determined.
Results: It was found that the malignant glioma cells could release an immunosuppressive factor in an autocrine fashion which was further identified as the transforming growth factor β,sub>2
