Original Article
DETECTION OF t(14 18) CHROMOSOMAL TRANSLOCATION IN PARAFFIN-EMBEDDED HEPATOCELLULAR CARCINOMA TISSUE BY IN SITU PCR
Abstract
Objective: To understand the relationship between (14; 18) chromosomal translocation and hepatocellular carcinoma.
Methods: Semi-nested in situ PCR (SNISPCR) technique was used to detected bcl-2/JH fusion gene in 40 cases of hepatocellular carcinoma (HCC).
Results: Bcl-2/JH fusion gene was detected in 10 of 40 HCC. There were no significant differences in bcl-2/JH fusion formation between histopathological grades and metastases (P>0.05).
Conclusion: By detecting bcl-2 fusion gene in HCC, we think that t(14; 18) chromosomal translocation is not a specific change in lymphoma, t(14; 18) chromosomal translocation may not an important cause in pathologensis of HCC.
Methods: Semi-nested in situ PCR (SNISPCR) technique was used to detected bcl-2/JH fusion gene in 40 cases of hepatocellular carcinoma (HCC).
Results: Bcl-2/JH fusion gene was detected in 10 of 40 HCC. There were no significant differences in bcl-2/JH fusion formation between histopathological grades and metastases (P>0.05).
Conclusion: By detecting bcl-2 fusion gene in HCC, we think that t(14; 18) chromosomal translocation is not a specific change in lymphoma, t(14; 18) chromosomal translocation may not an important cause in pathologensis of HCC.
