Original Articles
DETECTION OF APOPTOTIC CELLS AND IMMUNOHISTOCHEMICAL STUDY OF bcl-2 AND p53 GENE PROTEIN IN PRIMARY GASTRIC MUCOSA-ASSOCIATED LYMPHOID TISSUE (MALT) LYMPHOMA
Abstract
Objective: To identify the apoptotic cells in gastric MALT lymphoma and its relationship between bcl-2 and p53 gene expression.
Methods: TdT-mediated dUTP biotin Nick End labeling (TUNEL) and immunohistochemistry ABC method were used to display apoptotic cells and the gene protein expression of bcl-2 and p53 independently.
Results: Apoptotic indices (AI) in high-grade MALT lymphomas were significantly higher than in mixed-grade group and low-grade group (P<0.05). Bcl-2 was expressed in 83% of low-grade tumors, 61.6% of the median-grade tumors and 43.7% of high-grade tumors. An inverse correlation was observed between the expression of bcl-2 and apoptotic indices. Only 27 cases were p53 positive. The frequency of p53 positivity was significantly increased as the histologic grade advanced (P<0.05). There was also an inverse correlation between the expression of bcl-2 and p53.
Conclusion: Apoptosis may be important in tumors development and transmission, p53 and bcl-2 were important regulatory genes of apoptosis and may be associated with transformation from low- grade to highgrade lymphomas.
Methods: TdT-mediated dUTP biotin Nick End labeling (TUNEL) and immunohistochemistry ABC method were used to display apoptotic cells and the gene protein expression of bcl-2 and p53 independently.
Results: Apoptotic indices (AI) in high-grade MALT lymphomas were significantly higher than in mixed-grade group and low-grade group (P<0.05). Bcl-2 was expressed in 83% of low-grade tumors, 61.6% of the median-grade tumors and 43.7% of high-grade tumors. An inverse correlation was observed between the expression of bcl-2 and apoptotic indices. Only 27 cases were p53 positive. The frequency of p53 positivity was significantly increased as the histologic grade advanced (P<0.05). There was also an inverse correlation between the expression of bcl-2 and p53.
Conclusion: Apoptosis may be important in tumors development and transmission, p53 and bcl-2 were important regulatory genes of apoptosis and may be associated with transformation from low- grade to highgrade lymphomas.
