@article{CJCR936,
author = {Xu Ma and Qing-yun Zhang},
title = {Protein Microarrays for Quantitative Detection of PAI-1 in Serum},
journal = {Chinese Journal of Cancer Research},
volume = {24},
number = {3},
year = {2012},
keywords = {},
abstract = {Objective: Plasminogen activator inhibitor-1 (PAI-1), one crucial component of the plasminogen activator system, is a major player in the pathogenesis of many vascular diseases as well as in cancer. High levels of PAI-1 in breast cancer tissue are associated with poor prognosis. The aim of this study is to evaluate rigorously the potential of serum PAI-1 concentration functioning as a general screening test in diagnostic or prognostic assays.
Methods: A protein-microarray-based sandwich fluorescence immunoassay (FIA) was developed to detect PAI-1 in serum. Several conditions of this microarray-based FIA were optimized to establish an efficacious method. Serum specimens of 84 healthy women and 285 women with breast cancer were analyzed using the optimized FIA microarray.
Results: The median serum PAI-1 level of breast cancer patients was higher than that of healthy women (109.7 ng/ml vs. 63.4 ng/ml). Analysis of covariance revealed that PAI-1 levels of the two groups were significantly different (P},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/936}
}