@article{CJCR935,
author = {Ming Han and Hua-yu Deng and Rong Jiang},
title = {Effect of Trastuzumab on Notch-1 Signaling Pathway in Breast Cancer SK-BR3 Cells},
journal = {Chinese Journal of Cancer Research},
volume = {24},
number = {3},
year = {2012},
keywords = {},
abstract = {Objective: To investigate the effects and mechanisms of trastuzumab on Notch-1 pathway in breast cancer cells, recognizing the significance of Notch-1 signaling pathway in trastuzumab resistance.
Methods: Immunocytochemistry staining and Western blotting were employed to justify the expression of Notch-1 protein in HER2-overexpressing SK-BR3 cells and HER2-non-overexpressing breast cancer MDA-MB-231 cells. Western blotting and reverse transcription PCR (RT-PCR) were used to detect the activated Notch-1 and Notch-1 target gene HES-1 mRNA expression after SK-BR3 cells were treated with trastuzumab. Double immunofluorescence staining and co-immunoprecipitation were used to analyze the relationship of Notch-1 and HER2 proteins.
Results: The level of Notch-1 nuclear localization and activated Notch-1 proteins in HER2-overexpressing cells were significantly lower than in HER2-non-overexpressing cells (P},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/935}
}