@article{CJCR890,
author = {Jing Li and Wilfred Hing-Sang Wong and Shing Chan and James Chor-San Chim and Kenneth Man-Chee Cheung and Tsz-Leung Lee and Wing-Yan Au and Shau-Yin Ha and Albert Kwok-Wei Lie and Yu-Lung Lau and Raymond Hin-Suen Liang and Godfrey Chi-Fung Chan},
title = {Factors Affecting Mesenchymal Stromal Cells Yield from Bone Marrow Aspiration},
journal = {Chinese Journal of Cancer Research},
volume = {23},
number = {1},
year = {2012},
keywords = {},
abstract = {Objective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC).
Methods: We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles.
Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors },
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/890}
}