@article{CJCR3346,
author = {Tongming Zhu and Yiwen Shen and Qisheng Tang and Luping Chen and Huasong Gao and Jianhong Zhu},
title = {BCNU/PLGA microspheres: a promising strategy for the treatment of gliomas in mice},
journal = {Chinese Journal of Cancer Research},
volume = {26},
number = {1},
year = {2014},
keywords = {},
abstract = {Objective: To investigate the effects of BCNU/PLGA microspheres on tumor growth, apoptosis and chemotherapy resistance in a C57BL/6 mice orthotopic brain glioma model using GL261 cell line.
Methods: BCNU/PLGA sustained-release microspheres were prepared by the water-in-oil-in-water emulsion technique. GL261 cells were intracranially injected into C57BL/6 mouse by using the stereotaxical technology. A total of 60 tumor-bearing mice were randomly and equally divided into three groups: untreated control, PLGA treated, BCNU/PLGA treated. Magnetic resonance imaging (MRI) was taken to evaluate tumor volume. BCNU/PLGA sustained-release wafers were implanted in the treatment group two weeks after inoculation. Survival time and quality were observed. Specimens were harvested, and immunohistochemical staining was used to check the expression of Bax, Bcl-2, and O6-methylguanine-DNA methyltransferase (MGMT). Statistical methods was used for analysis of relevant data.
Results: BCNU/PLGA sustained-release wafers were fabricated and implanted successfully. There is statistical difference of survival time between the BCNU/PLGA treated group and control groups (P0.05).
Conclusions: Local implantation of BCNU/PLGA microspheres improved the survival quality and time of GL261 glioma-bearing mice significantly, inhibited the tumor proliferation, induced more cell apoptosis, and did not increase the chemotherapy resistance.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/3346}
}