@article{CJCR2825,
author = {Jing Xie and Zhen Chen and Luming Liu and Ping Li and Xiaoyan Zhu and Huifeng Gao and Zhiqiang Meng},
title = {shRNA-mediated Slc38a1 silencing inhibits migration, but not invasiveness of human pancreatic cancer cells},
journal = {Chinese Journal of Cancer Research},
volume = {25},
number = {5},
year = {2013},
keywords = {},
abstract = {Objective: Early metastasis is a major biological feature of pancreatic cancer. The current study examined whether silencing Slc38a1, a gene involved in energy metabolism, using short hairpin RNA (shRNA) could inhibit the growth, migration, and invasiveness of pancreatic cancer cells.
Methods: A series of Slc38a1 shRNAs were designed and cloned into the pGPU6/GFP/Neo vectors. An shRNA with the most efficacious inhibitory action on SCL38A1 expression (65% inhibition) upon screening in DH5α bacteria was used to transfect SW1990 human pancreatic cancer cells. Cell growth, migration, and invasiveness were examined using cell counting kit-8, Boyden chamber without and with Matrigel, respectively.
Results: Transfection of SW1990 cells with the SLCs38A1 shRNA significantly decreased the proliferation (P},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/2825}
}