@article{CJCR2618,
author = {Hua Huang and Zhaoquan Liao and Jiqun Huang and Wei Huang},
title = {ENZYMO- ROCKET ELECTROPHORETIC ASSAY AND CROSSED AFFINITY ENZYMOIMMUNOELECTROPHORESIS AND ITS APLICATION IN DIAGNOSIS OF PLIMARY LIVER CANCER},
journal = {Chinese Journal of Cancer Research},
volume = {7},
number = {2},
year = {2013},
keywords = {},
abstract = {A sensitive and accurate quantitative method of alphafetoprotein (AFP), enzymo-rocket dectrophoretie assay (EREA), was developed by introducing horseradish peroxidase-labeled anti-btmmn AFP antibody into rocket electrophoresis. The lower limit of quantitation by this method is about 10 ng/ml of AFP. The dose-nesponse curve covers a broader range of concentrations of AFP (10--4000 ng/ml) than RIA (0---400 ng/ml). 3fine accm-acy and precision is comparable to that of RIA (r=0.986). Sermn AFP measured in 100 patients with primary liver cancer by this method, 88% had levels over 25 ng/ml.
The crossed affinity enzymoimmtmoelectrophoresis is a combination of lentil lectin (LCA) affinity electrophoresis and enzymo-rocket electrophoresis, it has been possible to separate the AFP into two variants, LCA-reactive (LCA-R) and LCA-nonreactive (LCA-N) fractions. The advantages of this method are high sensitivity, rapid (6--7 h), and can be effectively used to differentiate the primary liver cancer and benign liver disease.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/2618}
}