@article{CJCR2480,
author = {Yinxiong Li and Muzhen Fan and Jingli Zhang and Zhiquan Liang},
title = {EXPRESSION OF c-myc GENE AND BIOSYNTHESIS OF BIOLOGICAL MACROMOLECULES IN ANTISENSE TRANSFECTANT HLR60 -- 9*},
journal = {Chinese Journal of Cancer Research},
volume = {8},
number = {4},
year = {2013},
keywords = {},
abstract = {The recombinant plasmid PGC was constructed for transcription unit of c-myc gene with diorientation in vitro, to make RNA probes for detection of c-myc mRNA and antisence RNA expression of tranfectant HLR60-9, which was obtained from HL60 cells transfected with inducible c-myc antisense RNA expression plasmid. The results from HLR60-9 cells induced by Cd2+ indicated that expression of c-myc antisense RNA increased with Cd2+ concentration and exposure time, while c-myc mRNA expression progressively reduced. Using immunohistochemical technique no c-myc P62 protein expression was detected. The incorporation of 3H-TdR, 3H-UR and 3HLeu revealed significant suppression of DNA, RNA and protein biosynthesis. It is suggested that the reversion changes previously reported in malignant phenotypes of HLR60-9 cells and the inhibition of macromolecular biosynthesis mentioned above were associated with the blockade of c-myc gene expression by its antisense RNA.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/2480}
}