@article{CJCR2341,
author = {Tengping Qi and Peiji Zhang and Shuguang Wei and Dong Chen and Ren Li and Wuru Wang},
title = {STUDY ON METASTASIS ASSOCIATED GENE SCREENED BY MONOCLONAL ANTIBODY HIL},
journal = {Chinese Journal of Cancer Research},
volume = {8},
number = {1},
year = {2013},
keywords = {},
abstract = {The cDNA expression libraries derived from a highly metastatic cell subline Anip-973 and from its parental cell line, low metastatic AGZY-83a were screened by monoclonal antibodies (MoAbs) seperately against these two cell lines. A positive clone (H4-D) from the Anip-973 cDNA library was isolated and its nucleotide sequence was determined. This clone contained 978 bp with an open reading frame of 318 bp encoding a polypeptide consisting of 106 amino acids. The H4-D cDNA sequence showed 85% homology with a human propionyI-CoA carboxylase ct-chain. In Western bloting analysis, the MoAb H4 recognized 2 bands (15 KDa and 27 KDa) of Anip-973 cell membrane protein. The mRNA expression of H4-D was higher in Anip-973 cells than that in AGZY-83a cells. The metastatic potential of Anip-973 cells was markedly decreased after being pretreated with MoAb H4. The above findings indicated that H4-D has a certain relationship with the metastatic phenotype of Anip-973 cells.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/2341}
}