TY  - JOUR
AU  - Zhang, Weiping
AU  - Cao, Xueta
AU  - Tao, Qun
AU  - Hamada, Hirofumi
PY  - 2013
TI  - CONSTRUCTION AND EXPRESSION OF THE REPLICATIONDEFICIENT ADENONIRUS VECTOR OF HUMAN GM-CSF*
JF  - Chinese Journal of Cancer Research; Vol 9, No 4 (December 19, 1997): Chinese Journal of Cancer Research
Y2  - 2013
KW  - 
N2  - The full-length cDNA encoding human Granuiocyte-macrophage colony-stimulating factor (GMCSF) was cloned by RT-PCR, placed under the control of CMV promoter, and inserted into adenovirus vector of El-substitution type, pAxlcw. Subsequently, the cassette cosmid was cotransfected into 293 cells together with EcoT22I-digested Ad5-TPC, and the replication-deficient recombinant adenoviruses(Ad) of human GM-CSF were generated efficiently by homologous recombination, with the titers of 1.51×109pfu/ml. 48 hours after infection with prepared human GM-CSF recombinant adenoviruses in vitro, HeLa cells and primary human skin fibroblasts expressed high levels of human GM-CSF (80~400ng/106cells/24hr). These suggest that the recombinant Ad of human GM-CSF prepared by COS/TPC method is effective in mediating GM-CSF gene transfer and might be used in cancer gene therapy.
UR  - https://cjcr.amegroups.org/article/view/2315