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CONSTRUCTION AND EXPRESSION OF THE REPLICATIONDEFICIENT ADENONIRUS VECTOR OF HUMAN GM-CSF*

   
@article{CJCR2315,
	author = {Weiping Zhang and Xueta Cao and Qun Tao and Hirofumi Hamada},
	title = {CONSTRUCTION AND EXPRESSION OF THE REPLICATIONDEFICIENT ADENONIRUS VECTOR OF HUMAN GM-CSF*},
	journal = {Chinese Journal of Cancer Research},
	volume = {9},
	number = {4},
	year = {2013},
	keywords = {},
	abstract = {The full-length cDNA encoding human Granuiocyte-macrophage colony-stimulating factor (GMCSF) was cloned by RT-PCR, placed under the control of CMV promoter, and inserted into adenovirus vector of El-substitution type, pAxlcw. Subsequently, the cassette cosmid was cotransfected into 293 cells together with EcoT22I-digested Ad5-TPC, and the replication-deficient recombinant adenoviruses(Ad) of human GM-CSF were generated efficiently by homologous recombination, with the titers of 1.51×109pfu/ml. 48 hours after infection with prepared human GM-CSF recombinant adenoviruses in vitro, HeLa cells and primary human skin fibroblasts expressed high levels of human GM-CSF (80~400ng/106cells/24hr). These suggest that the recombinant Ad of human GM-CSF prepared by COS/TPC method is effective in mediating GM-CSF gene transfer and might be used in cancer gene therapy.},
	issn = {1993-0631},	url = {https://cjcr.amegroups.org/article/view/2315}
}