@article{CJCR2307,
author = {Quanxing Wang and Xuetao Cao and Jing Mi and Weiping Zhang},
title = {IMMUNOLOGICAL CHARACTERISTICS OF THE LEUKEMIA CELLS TRANSFECTED WITH ONCOSTATIN M GENE BY RECOMBINANT ADENOVIRUS1},
journal = {Chinese Journal of Cancer Research},
volume = {9},
number = {4},
year = {2013},
keywords = {},
abstract = {In the present study, FBL-3 murine erythroleukemia cells were transfected with human OSM(hOSM) gene by recombinant adenovirus, then the immunological properties of hOSM gene-transfected FBL-3 celIs(FBL-3-OSM+) were investigated. 4 hours after transfection with hOSM gene, hOSM could be detected in the supernatant of FBL-3-OSM+ cells and hOSM secretion peaked at 24 h. The proliferation of FBL-3-OSM+ cells was inhibited markedly. The clonal formation of FBL-3-OSM+ cells was suppressed more obviously in comparison with wild-type FBL-3 cells when analysed in clonal argar culture. Flow cytometry analysis showed that FBL-3-OSM+ cells expressed higher levels of Fas protein, B7 and ICAM-1 molecules.FBL-3-OSM+ cells also expressed higher level of MHC class I molecules(H-2K b) but remained unchanged in expression of MHC class II molecules (Ia). CDI4, which is a specific marker of monocyte/macrophage and not expressed on the wild-type FBL-3 cells, was also detected on the surface of FBL-3-OSM+ cells. The results suggested that OSM gene transfer could increase the immunogenicity of FBL-3 cells and promote their differentiation into macrophage-like cells. The data outline a promising approach to OSM gene therapy of leukemia mediated by recombinant adenovirus.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/2307}
}