@article{CJCR2304,
author = {Dianwen Ju and Xuetao Cao and Tao Wan and Weiping Zhang and Qun Tao and Yizhi Yu and Guoyou Chen},
title = {ANTITUMOR EFFECT OF GRANULOCYTE-MACROPHAGE COLONY- STIMULATING FACTOR (GM-CSF)-GENE ENCODED VACCINIA MELANOMA ONCOLYSATE AND ITS IMMUNOLOGICAL MECHANISMS1},
journal = {Chinese Journal of Cancer Research},
volume = {9},
number = {4},
year = {2013},
keywords = {},
abstract = {Vaccinia melanoma oncolysate (VMO) prepared by infecting B16F10 melanoma cells with recombinant vaccinia virus encoding murine GM-CSF gene was tested for its therapeutic effect on the preestablished melanoma. C57BL/6 mice were inoculated s.c. with 1 × l05 B16F10 melanoma cells and received s.c. administration with VMO prepared with GM-CSF gene-encoded vaccinia virus(GM-CSFVMO), VMO prepared with thymidine kinase gene-deficient vaccinia virus(TKVMO), B16F10 melanoma oncolysate(BMO), or PBS 3 days after tumor inoculation. The same treatment was bolstered one week later. The results demonstrated that GM-CSFVMO treatment significantly inhibited the growth of subcutaneous tumor and prolonged the survival period of tumor-bearing mice. Further study elucidated that cytotoxicity of PBL and splenocytes towards B16F10 increased obviously after treatment with GM-CSFVMO, but NK activity remained unchanged. These results suggest that the tumor oncolysate vaccine prepared with GM-CSF gene-encoded vaceinia virus might exert potent therapeutic effect on the preestablished tumor through the efficient induction of specific antitumor immune response of the host.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/2304}
}