@article{CJCR1825,
author = {Yanping Sun and Xuetao Cao and Quanxing Wang and Yuanhe Wang and Jinghua Shi},
title = {ANTITUMOR EFFECT OF INTRATUMORAL INJECTION OF LIPOSOME-ENCAPSULATED G-CSF GENE AND IN SITU BIOLOGICAL CHARACTERISTICS OF THE TREATED TUMOR CELLS*},
journal = {Chinese Journal of Cancer Research},
volume = {10},
number = {1},
year = {2013},
keywords = {},
abstract = {In order to investigate the antitumor effects of the in vivo G-CSF gene therapy mediated by liposome and its mechanisms, human G-CSF gene was encapsulated into liposome and was directly injected into tumor mass of C- 26 colon adenocarcinoma-bearing mice. After direct intratumoral injection of liposome encapsulated G-CSF DNA, the subcutaneous tumor growth was dramatically inhibited and the survival time was prolonged significantly. Tumor regression could be observed in about 30% of C-26-bcaring mice. By the analysis of the antitumor mechanisms, we found that anti-G418 (600ug/ml) clone could be selected from the tumor cells freshly separated from the treated C-26 tumor mass, and secretion of GCSF in the supernatant could be detected. Northern-blot also confirmed the expression of hG-CSF by the tumor cells. Higher expressions of MHC class I(H-2kd) molecule and ICAM-1 on the tumor cells could be observed. The results demonstrated that liposome can effectively transfect G-CSF gene into tumor cells in situ, and then increase the immunogeulcity of the tumor cells which may contribute to the activation of the local antitmnor immune responses effectively.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/1825}
}