@article{CJCR1604,
author = {Jingyan Xu and Min Zhou and Jing Wang and Qiguo Zhang and Yong Xu and Yueyi Xu and Qian Zhang and Xihui Xu and Hui Zeng and Jian Ouyang},
title = {Gambogic acid induces mitochondria-dependent apoptosis by modulation of Bcl-2 and Bax in mantle cell lymphoma JeKo-1 cells},
journal = {Chinese Journal of Cancer Research},
volume = {25},
number = {2},
year = {2013},
keywords = {},
abstract = {Objective: To study the mechanisms in gambogic acid (GA) -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro.
Methods: the proliferation of GA-treated JeKo cells was measured by CCK-8 assay and ki-67 immunocytochemical detection. Apoptosis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3, -8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blot.
Results: GA inhibited cell growth in a time- and dose- dependent manner. GA induces apoptosis in JeKo-1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bcl-2 and Bax without cell cycle arresting.
Conclusions: GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle.},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/1604}
}