TY  - JOUR
AU  - Xiang, Zhi-fu
AU  - Lu, Yu-ying
AU  - Lai, Yong-rong
AU  - Chen, Yan
AU  - Li, Hui-yu
AU  - Zou, Ping
PY  - 2013
TI  - Bcl-2 GENE REARRANGEMENT DETERMINED BY PCR AS A MEAN TO DETECT MINIMAL RESIDUAL DISEASE IN MALIGNANT LYMPHOMAS
JF  - Chinese Journal of Cancer Research; Vol 11, No 1 (March 11, 1999): Chinese Journal of Cancer Research
Y2  - 2013
KW  - 
N2  - Objective: To develop a sensitive method to detect minimal residual disease and to elucidate the significance of bcl-2 gene rearrangement in diagnosis and treatment of malignant lymphoma.   Methods: Using polymerase chain reaction (PCR) to detect bcl-2 gene rearrangement and using serial dilution method to define the sensitivity of PCR.   Results: In 9 different malignant lymphoma cell lines, Su-DHL-4 and Su-DHL- 6 were shown bcl-2(MBR)/JH rearrangement, the sensitivity of PCR was 1:10 s. In 16 patients with follicular lymphoma, the peripheral blood and bone marrow were PCR positive in 4 cases both at initial diagnosis and after complete remission.   Conclusion: Detection of bcl-2 gene rearrangement by PCR provides a sensitive and specific assay of minimal residual disease. It is helpful to improve staging of disease, prognosis and evaluation of the treatment results.
UR  - https://cjcr.amegroups.org/article/view/1582