@article{CJCR1521,
author = {Jian-li Chen and Sen Jiang and Rui-fang Yang and Fu-sheng Liu and Xiao-ming Sun},
title = {MECHANISM OF DRUG RESISTANCE AND REVERSAL WITH LIGUSTRA-ZINE AND CYCLOSPORIN A IN CISPLATIN-INDUCED HUMAN EPITHELIAL OVARIAN CANCER RESISTANT CELL LINE 3AO/CDDP},
journal = {Chinese Journal of Cancer Research},
volume = {12},
number = {3},
year = {2013},
keywords = {},
abstract = {Objective: To investigate the mechanism of resistance and reversal effect of ligustrazine and cyclosporin A in cisplatin-induced multidrug resistance ovarian cancer cell line 3Ao/cDDP.
Methods: Using the corresponding dose calculated from clinical chemotherapy at 30 mg cisplatin per cycle, we established 3Ao/cDDP with 3Ao exposed at regular intervals and repeatedly to high-level concentration of cisplatin at 10 llg/ml for 24 hours each time. Expressions of LRP, MRP, P-gp, GSTπ and TopoII were quantitatively detected with FCM. For drug resistance reversal, cyclosporin A and ligustrazine were administered singly or in combination at the maximal dose without cytotoxicity. Inhibition rates were determined by MTT assay.
Results: 3Ao/cDDP was established after 4.5 months, with resistance factor 1.6 which was similar to clinical resistance degree. Low expression levels of MRP and P-gp were found in both 3Ao and 3Ao/cDDP (P>0.05), and LRP and GSTx expression levels in 3Ao/cDDP were significantly higher than those in 3Ao (P},
issn = {1993-0631}, url = {https://cjcr.amegroups.org/article/view/1521}
}