How to cite item

CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTOR GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR GENE

   
@article{CJCR1493,
	author = {Qiu-hong Zheng and Tian-rong Zheng and Yun-qing Xie and Lin Lu and Hui Chen},
	title = {CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTOR GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR GENE},
	journal = {Chinese Journal of Cancer Research},
	volume = {12},
	number = {2},
	year = {2013},
	keywords = {},
	abstract = {Objective: To construct the eukaryotic expression vector that express human granulocyte-macrophage colony-stimulating factor (hGM-CSF) gene for making highly express in mammalian cells. 
Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR), and then directionally subcloned into the HindIII and EcoRI site on the pcDNA3.1 plasmid, which was controlled by the CMV promoter, to form the recombinant expressing vector pcDNA3.1-GM-CSF. 
Results: The PCR amplification was identified and the sequence was analyzed, the results showed that hGM-CSF was properly inserted into the vector and the sequence was correct.},
	issn = {1993-0631},	url = {https://cjcr.amegroups.org/article/view/1493}
}